Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation.

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Tác giả: Ingo Burtscher, Anita Hofmann, Heiko Lickert, Nicole Katarina Rogers, Eunike Sawitning Ayu Setyono

Ngôn ngữ: eng

Ký hiệu phân loại: 332.152 International banks for monetary stabilization and balance of payments

Thông tin xuất bản: England : Stem cell research , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 710684

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Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.

1. Generation
2. Of
3. Arx

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