Mercury (Hg) contamination affects all ecosystems worldwide. Its deleterious effects on wildlife and humans encompass a diversity of impacts from individual to population levels. In the present study, we quantified Hg concentration across various tissues (blood, brain, muscle, and toe) of green toads (Bufotes viridis) and investigated the use of toe clips as a proxy of Hg concentration in internal tissues, including the brain. Our results show distinct patterns of Hg contamination across tissues, with the highest Hg concentration in the blood with 1.496 ± 0.772 µg.g