The microbial detoxification method demonstrates significant potential for detoxifying feed contaminated with mycotoxins, but the application of degrading bacteria in mammals was rarely investigated. In this study, the effects of dietary zearalenone on the growth performance, reproductive performance, reproductive organs, hormone levels of rats and the toxicity alleviation of Bacillus subtilis MZ18 were studied. From gestation day 0-20, pregnant SD rats received either a normal diet or a diet supplemented with zearalenone at a dose of 20 mg/kg⋅BW, and with or without supplementation of Bacillus subtilis MZ18 culture. In addition to the negative effects on the growth performance of dietary zearalenone, we found that the ovarian weight was increased, the number of follicles and granulosa lutein cells in the corpus luteum was reduced, and the placental tissue exhibited an enlarged interstitial space and signs of stasis. Further analysis revealed a reduction in serum levels of LH, FSH, and E