Mangroves are crucial for supporting coastal communities, benefiting both humans and animals. Their intricate root systems grasp the soil, mitigating erosion and dampening wave impact. As a result, they shield and fortify shorelines that would otherwise erode. This study focuses on the isolation of endophytic bacteria from the stem of Bruguiera gymnorrhiza. Molecular analysis using 16S rDNA gene sequencing identified the isolate as Bacillus subtilis (BG1). Its hydrolytic enzyme production capabilities were assessed, revealing its ability to produce cellulase, amylase, protease and L-asparaginase. The isolate also demonstrated various plant growth promoting traits including ammonia production, indole 3- acetic acid (IAA) production, hydrogen cyanide (HCN) production, phosphate solubilization, and ACC deaminase activity. IAA and organic acid production were quantified using Liquid Chromatography - Mass Spectrometry (LC-MS/MS), with B. subtilis producing 86.32 ± 0.64 μg mL