Microplastics (MPs) are emerging environmental pollutants which represent a serious threat to ecosystems and human health and have received significant attention from the global community. Currently, a growing number of studies have found the presence of MPs in groundwater. This study exhaustively reviewed varying degrees of recent publications in Web of Science database and investigated the characteristics of MPs (concentration, types, sizes and shapes) in groundwater ecosystems, their migration characteristics, and interactions with co-occurring contaminants. Results suggested that current global research on MPs in groundwater has primarily focused on countries such as India, South Korea, China, Italy and United States. Pollution levels of MPs in groundwater show significant variability, ranging from 0 to 6832 n/L. The predominant plastic polymer types include PP, PE, PS, PA, PET and PVC. The sources of MPs in groundwater are primarily classified as associated with natural processes and anthropogenic activities. The physical, chemical and biological properties can influence the migration of MPs into groundwater. Furthermore, MPs can act as carriers, interacting with co-occurring contaminants, thereby enhancing their migration and toxicity, potentially posing a threat to groundwater ecosystems and human health. Consequently, the major challenges and associated recommendations for forthcoming research on MPs in groundwater are proposed.