INTRODUCTION: The authors evaluated factors associated with neonatal hepatitis B vaccination (HepB), including prenatal vaccinations, race, ethnicity, neonatal disposition, parity, and maternal age to identify potential areas of engagement to improve maternal and child health. METHODS: The authors conducted a retrospective cohort study of patients who received prenatal care and delivered at an academic tertiary care hospital in central Pennsylvania from 2015-2020. A multiple logistic regression model was used to assess factors associated with newborn receipt of HepB. RESULTS: Prenatal vaccination was significantly ( CONCLUSION: Prenatal vaccination was significantly associated with neonatal in-hospital HepB vaccine uptake.