MicroRNAs are endogenous non-coding small RNAs composed of about 22 nucleotides, which are widely found in eukaryotic cells and regulate gene expression at the post-transcriptional level through complementary pairing with target genes, leading to mRNA degradation or translation inhibition. MiR-34 family is a highly conserved miRNA family during evolution. Recent studies have found that members of the miR-34 family are involved in regulating biological processes such as aging, ciliogenesis, and immunity. To have a more comprehensive understanding of miR-34 family, this paper reviewed the functional evolution of miR-34 family, and provided a reference for further research on the related functions of miR-34.