Phthalic acid esters (PAEs) are widely used due to their advantageous properties, which enhance the durability, flexibility, and transparency of plastic products. Nanomaterials are also commonly used in plastic additives and agricultural fertilizers. However, both are easy to fall off, diffuse, and release into the environment during production, use, and disposal. The adsorption and transportation of PAEs by nanomaterials may jointly affect soil health. However, less attention is paid to the soil microorganisms caused by co-exposure between PAEs and nanomaterials, especially mediated by earthworms. The present study investigated the effects of BBP (1 mg kg