This investigation aimed to examine the synergistic effects of fresh royal jelly and local wild Artemisia monosperma leaf extract as antibacterial, antioxidant, antibiofilm, and anti-Alzheimer activity. Gas chromatography-mass spectrometry (GC-MS) identified 16 compounds in Artemisia monosperma, including tricosadiynoic acid, hexadecenoic acid, octadecenoic acid. In contrast, fresh royal jelly contained 13 compounds, including dodecanoic acid, octadecynoic acid, hexadecenoic acid, heptatriacotanol, and their derivatives. The Artemisia monosperma extract exhibited significant antioxidant activity in the DPPH assay, with IC