Placenta-derived factors contribute to human iPSC-liver organoid growth.

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Tác giả: Erica Carolina, Yoichi Furukawa, Toshiharu Kasai, Yoshiki Kuse, Shinya Matsumoto, Takayoshi Oba, Takashi Okumura, Tomomi Tadokoro, Hideki Taniguchi, Naoki Tanimizu, Syusaku Tsuzuki, Yasuharu Ueno, Soichiro Yamabe, Kiyoshi Yamaguchi

Ngôn ngữ: eng

Ký hiệu phân loại: 631.847 Biological methods of soil nitrification

Thông tin xuất bản: England : Nature communications , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 711377

Organoids derived from human induced pluripotent stem cells (hiPSC) are potentially applicable for regenerative medicine. However, the applications have been hampered by limited organoid size and function as a consequence of a lack of progenitor expansion. Here, we report the recapitulation of progenitor expansion in hiPSC-liver organoids based on the analysis of mouse development. Visualization of blood perfusion and oxygen levels in mouse embryos reveals a transient hypoxic environment during hepatoblast expansion, despite active blood flow. During this specific stage, the placenta expresses various growth factors. Human and mouse placenta-liver interaction analysis identifies various placenta-derived factors. Among them, IL1α efficiently induces the growth in hiPSC-liver organoids as well as mouse fetal livers following progenitor expansion under hypoxia. Furthermore, subsequent oxygenation demonstrates that progenitors expanded by IL1α contribute to hiPSC-liver organoid size and function. Taken together, we demonstrate that treatment with the placenta-derived factor under hypoxia is a crucial human organoid culture technique that efficiently induces progenitor expansion.
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