Asymmetry in centrosome maturation revealed through AIR-1 dynamics in the early Caenorhabditis elegans embryo.

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Tác giả: Adriana T Dawes, Natalia Kravtsova, Shayne M Plourde

Ngôn ngữ: eng

Ký hiệu phân loại: 633.14 *Rye

Thông tin xuất bản: England : Scientific reports , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 711482

Centrosomes are critical organelles associated with the nucleus, consisting of a pair of centrioles surrounded by a cloud of pericentriolar material. They serve as key nucleation sites for microtubule arrays and are essential for positioning the nucleus prior to cell division, but mechanisms for ensuring proper centrosome positioning are not well understood. Previous research has identified asymmetries in microtubule arrays nucleated by centrosomes prior to cell division, including during the first cell cycle in Caenorhabditis elegans, as playing a critical role in centrosome positioning, however the origin of this asymmetry remains unclear. To explore potential sources of centrosome asymmetry, we developed a mathematical model of centrosome maturation, distinct from prior models by not presuming any inherent symmetry or asymmetry. We determined model parameters using in vivo data on the recruitment and recovery of GFP-tagged AIR-1 (Aurora kinase A) in early C. elegans embryos, enabling precise parameter estimation. Our results reveal an intrinsic asymmetry in centrosome dynamics that highlights the potential for variability in physical centrosome characteristics. These dynamics produce highly consistent microtubule arrays, independent of specific structural details. These findings offer novel insights into centrosome behavior and positioning during cell division and early embryonic development.
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