Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15-25 Å.

• Tác giả: Charlotte A Dodson, Paul Girvan, Liming Ying
• Ngôn ngữ: eng
• Ký hiệu phân loại: 994.03 Period of development of self government, 1851-1901
• Thông tin xuất bản: England : Chemical communications (Cambridge, England) , 2025
• Bộ sưu tập: NCBI
• ID: 712146

Tóm tắt

Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.