BACKGROUND: Endometrial cancer (EC) is the fourth most commonly diagnosed cancer among women in the US and the fifth leading cause of cancer death in this population. The FBXW7 tumor suppressor gene is frequently mutated in all molecular subtypes of EC. The encoded protein is part of a ubiquitin ligase complex that targets substrate proteins for ubiquitination and, in most instances, proteasome-mediated degradation. AIMS: The purpose of this investigation was to identify the proteomic changes associated with endogenous FBXW7 mutations in EC. MATERIALS & METHODS: Quantitative LC-MS/MS was used to identify significant (p <
0.05) differences in the proteomes and phosphoproteomes of two FBXW7-mutated EC cell lines, HEC-1-B RESULTS: Analysis of LC-MS/MS results identified 397 total proteins and/or phosphoproteins with significantly different levels in both HEC-1-B CONCLUSION: This study provides novel insights into the proteomic and phosphoproteomic effects of the endogenous FBXW7