Nanozymes, a class of nanomaterials mimicking natural enzymatic functions, have gained significant attention due to their exceptional biocatalytic properties and wide-ranging applications in biosensing. The total antioxidant capacity (TAC) can serve as a crucial parameter for assessing food quality, guiding dietary choices, and monitoring health conditions. In recent years, various nanomaterials with peroxidase (POD)-like and oxidase (OXD)-like activity have been widely used for TAC determination. This review discusses the enzyme-mimicking catalytic activities of nanozymes related to TAC determination, the construction principles of nanozyme-based TAC sensors and systematically classifies the application of nanozyme sensors in TAC determination. Furthermore, the potential opportunities and challenges in the development of nanozyme-based sensors are evaluated, aiming to provide valuable insights for researchers in related fields.