This study aimed to investigate the potential reduction on the sensitization of sesame protein Ses i 3 through ultrasound-assisted glycation. Ses i 3 was extracted and purified using an immunoaffinity column, and the allergenicity changes of Ses i 3 were assessed by a comprehensive strategy, and T cell polarization was also assessed in vivo. Results showed ultrasound-assisted glycation treated Ses i 3 was more easily digestible
and the cell degranulation model showed the histamine, tryptase, and β-hexosaminidase induced by ultrasound-assisted glycation treatment were significantly decreased
besides, the serological results demonstrated that a notable decrease in the binding ability of immunoglobulin E (IgE) and IgG
finally, a BALB/c mice model demonstrated an alleviation of allergic responses induced by ultrasonic-assisted glycation treatment. Meanwhile, the results in vivo also found that ultrasonic-assisted glycation treated Ses i 3 induced enhanced Helper T cell (T