Feature-based molecular networking analysis combined with bioassay-guided method was applied to afford two previously undescribed trichothecenes derivatives (1-2), together with eleven trichothecenes derivatives (3-13) and three β-resorcylic macrolides (14-16) from an endophytic fungus Fusarium equiseti Z8. The new structures were elucidated by using comprehensive spectroscopic analyses of NMR, HR-ESI-MS, and ECD calculations. MS