Single-cell RNA sequencing and cell-cell communication analysis reveal tumor microenvironment associated with chemotherapy responsiveness in ovarian cancer.

• Tác giả: Ningxuan Chen, Xiaoyan Jiang, Xiaoyi Liu, Xin Luo, Qinglv Wei, Lingcui Xie, Jing Xu, Ping Yi
• Ngôn ngữ: eng
• Ký hiệu phân loại: 615.8043 Specific therapies and kinds of therapies
• Thông tin xuất bản: Italy : Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico , 2025
• Bộ sưu tập: NCBI
• ID: 715180

Tóm tắt

BACKGROUND: To investigate the impact of the tumor microenvironment (TME) on the responsiveness to chemotherapy in ovarian cancer (OV). METHODS: We integrated single cell RNA-seq datasets of OV containing chemo-response information, and characterize their clusters based on different TME sections. We focus on analyzing cell-cell communication to elaborate on the mechanisms by which different components of the TME directly influence the chemo-response of tumor cells. RESULTS: scRNA-seq datasets were annotated according to specific markers for different cell types. Differential analysis of malignant epithelial cells revealed that chemoresistance was associated with the TME. Notably, distinct TME components exhibited varying effects on chemoresistance. Enriched SPP1 CONCLUSION: Our study indicates that the non-tumor cell components of the TME (e.g. SPP1