Tear levels of apoptotic, matrix-degrading and antioxidant biomarkers in patients with and without keratoconus: A cross sectional study.

• Tác giả: Danilo Alunni-Fegatelli, Umberto Camellin, Francesco Franchina, Giuseppe Lombardo, Marco Lombardo, Rita Mencucci, Anna Maria Roszkowska, Sebastiano Serrao, Annarita Vestri
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Contact lens & anterior eye : the journal of the British Contact Lens Association , 2025
• Bộ sưu tập: NCBI
• ID: 715591

Tóm tắt

 PURPOSE: To assess the tear levels of a set of apoptotic, matrix-degrading and antioxidant biomarkers, including Metalloproteinase 9 (MMP9), High Mobility Group Box 1 (HMGB1) and Superoxide Dismutase 3-Extracellular (SOD3). METHODS: Sandwich-ELISA commercial kits were used to test the expression of the three tear biomarkers in the lacrimal fluid of eligible participants. Linear logistic regression analysis was performed todetermine whether the set of tear biomarkers could be associated with clinically manifest keratoconus. ROC curve analysis using 10-fold cross-validation was performedto evaluate the prediction accuracy of the model. RESULTS: Eighty-one participants aged 30-48 years old were enrolled in this study
  48 were patients with keratoconus and 33 were age-matched healthy subjects. The linear combination of the three tear biomarkers levels (AUC = 0.811
  CI 95 %: 0.712-0.911) accurately indicated the existence of keratoconus
  higher levels of MMP9 (Odd Ratio: 1.069
  CI 95 %: 1.029-1.130) and HMGB1 (OR: 1.011
  CI 95 %: 1.003-1.022) and lower levels of SOD3 (OR: 0.994
  CI 95 %: 0.989-0.997) were significantly associated with a higher probability of keratoconus. CONCLUSION: Multivariable analysis of the set of tear levels of MMP9, HMGB1 and SOD3 biomarkers confirmed a chronic state of inflammation in the ocular surface of patients with keratoconus.