Automation and miniaturization of high-throughput qPCR for gene expression profiling.

• Tác giả: Iman Al Azwani, Muna Al Hashmi, Alia Al Massih, Basirudeen Syed Ahamed Kabeer, Mahesh Kumar Reddy Kalikiri, Harshitha Shobha Manjunath, Rebecca Mathew, Santhi Raveendran, Asma Saeed, Sara Tomei
• Ngôn ngữ: eng
• Ký hiệu phân loại: 133.594 Types or schools of astrology originating in or associated with a
• Thông tin xuất bản: United States : SLAS technology , 2025
• Bộ sưu tập: NCBI
• ID: 716109

Tóm tắt

 Quantitative PCR (qPCR) is a technique commonly employed in laboratories and core facilities. In our previous study, we had shown the possibility to automate steps in a panel-specific gene expression workflow by pairing Mosquito HV with BioMark HD. Here we aimed to automate the full workflow and explore miniaturization capabilities. Each step of the gene expression workflow was scripted on Mosquito HV genomics software. We performed three different automated runs: i. Replicates of a Reference RNA sample (obtained by pooling RNA isolated from 10 healthy individuals) were run on an immunology gene expression panel. We tested the full reaction (FR) and three miniaturization conditions, namely: 1.5x, 2.5x and 5x
  the data obtained from the automated FR replicates was compared to the data obtained from the manual processing
  ii. Biological RNA samples (isolated from n = 45 individuals) were run as FR and 1.5x on the immunology gene expression panel
  iii. Biological RNA samples (isolated from n = 45 individuals) were run as FR and 1.5x on a pregnancy gene expression panel. The expression of each gene was calculated using the 2