Enhancement of differentiation and mineralization of human dental pulp stem cells via TGF-β signaling in low-level laser therapy using Er:YAG lasers.

• Tác giả: Risako Chiba-Ohkuma, Noriyasu Hosoya, Takeo Karakida, Kazuyuki Kobayashi, Kazuo Onuma, Shunjiro Yamakawa, Yasuo Yamakoshi, Ryuji Yamamoto, Yasushi Yamazaki, Ryo Yoshida
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : Journal of oral biosciences , 2025
• Bộ sưu tập: NCBI
• ID: 716416

Tóm tắt

 OBJECTIVES: Low-level laser therapy (LLLT) using an erbium-doped yttrium aluminum garnet (Er:YAG) laser provides a non-invasive approach applicable to various dental treatments. Here, we investigated the effects of Er:YAG laser irradiation on human dental pulp stem cells (hDPSCs) in an in vitro experiment. METHODS: The hDPSCs were categorized into four groups: laser-irradiated with activators (VLT: activated vitamin D RESULTS: Cell proliferation was similar between the LLLT(+)-only and control groups. The ALP activity was significantly higher in LLLT(+)VLT group than in LLLT(-)VLT group (p <
  0.05)
  however, it was suppressed by TGF-β signaling inhibitors. Western blotting showed enhanced SMAD3 phosphorylation in the LLLT(+)VLT group. The mineralization nodules and mRNA levels of matrix vesicle marker genes were significantly higher in LLLT(+)VLT group, and the nodules were partially composed of hydroxyapatite. The hard tissue formation marker gene expression in LLLT(+)VLT group was significantly higher (p <
  0.05) than that in the LLLT(+)-only and control groups
  however, it was unchanged or suppressed compared with that in LLLT(-)VLT group. CONCLUSIONS: LLLT using an Er:YAG laser, combined with VLT, may promote the differentiation of hDPSCs into hard tissue-forming cells and enhance mineralization.