Fabricating Silver Nanoparticles with Antidiabetic d-Pinitol to Restrict Amyloid Formation of Insulin.

• Tác giả: Bibin Gnanadhason Anand, Masihuzzaman Ansari, Karunakar Kar, Om Prakash Mahato, Nishant Mishra, Shikha Mittal, Kailash Prasad Prajapati
• Ngôn ngữ: eng
• Ký hiệu phân loại: 553.453 Tin
• Thông tin xuất bản: United States : ACS applied bio materials , 2025
• Bộ sưu tập: NCBI
• ID: 716976
• Chủ đề: 1. Fabricating; 2. Silver; 3. Nanoparticles

Tóm tắt

In this study, we synthesized silver nanoparticles functionalized with antidiabetic d-pinitol molecules and investigated their anti-amyloid effect on Insulin aggregation. Our results show that these functionalized nanoparticles effectively inhibit both spontaneous and seed-induced amyloid aggregation of Insulin in a dose-dependent manner. The d-pinitol-functionalized silver nanoparticles interact directly with the aggregation-prone regions of Insulin's partially unfolded structures, as demonstrated by quenching and computational experiments. Analysis of docking and simulation data suggests that antiamyloid activity of d-pinitol functionalized AgNPs is due to a strong Insulin-nanoparticle interaction, facilitated via hydrogen bonds. These interactions disrupt the Insulin monomer-dimer equilibrium and prevent the formation of toxic amyloid structures. The results could lead to the development of d-pinitol-based Insulin-stabilizing nanoformulations with potential antidiabetic properties.