Microorganisms (e.g., bacteria, fungi, and viruses) add indispensable functionality to a range of electrospun polymer materials and devices. The optimal distribution of bioactive agents on either the interior or exterior of the fiber is application-specific. Current microbe surface immobilization strategies and core-confinement techniques continue to pose a number of challenges. Here, we explore a simple strategy, utilizing electrostatic forces, to control the migration and surface concentration of the M13 bacteriophage within near-field electrospun poly(vinyl alcohol) (PVA) microfibers. Both the surface charge of the electrospun virus and the applied electric field polarity altered microbe placement. When doped with Rhodamine 6G (R6G), the circular microfiber cross sections formed active whispering gallery mode (WGM) resonators. These relatively high-quality (