Memes have become a distinct form of language on the virtual media and a more frequent way of nonverbal communication, especially among Generation Z. Memes can be used to convey information, humor, criticism, or sarcasm, usually as a form of expression of one's feelings. Existing research on memes is predominated by humor as a coping mechanism for stress. However, the negative impact of memes on Gen Z remains an under-researched area. Since the young generation places a high value on virtual communication and how they are perceived online, they are likely to be affected psycho-physiologically, at times becoming victims of depression due to the negative impact of memes at their workplace or homes. This study used the validated Patient Health Questionnaire and conducted a survey among Gen Z workforce across the fintech, information technology, manufacturing, and retail sectors. 528 responses were analyzed using SPSS and SmartPLS 4. This study is perhaps the first of its kind to examine the gender-based psycho-physiological impact of memes as virtual nonverbal communication, on Gen Z. This study is unique as it examines the psycho-physiological impact of memes on male and female Generation Z workers. The analysis revealed in relation to memes, among the Gen Z workforce, females exhibit more hardiness as compared to males. This study has practical implications for managers of this young workforce, as it provides them insights on the negative influence on virtual nonverbal communication and the importance of sensitizing their young workers about the negative psycho-physiological impact of memes.