Avian metapneumovirus (aMPV) is a viral pathogen that mainly causes respiratory signs and drops in egg production in turkeys, chickens, and ducks. Here, an aMPV subgroup C (aMPV/C) strain, designated GX22-01, was isolated and identified from severe respiratory disease in broiler breeder chickens in 2022 in Jiangsu, China, as evidenced by indirect immunofluorescence and western blotting using specific anti-viral protein antibodies and by sequence analysis of viral nucleoprotein (N) gene. N gene sequencing indicated that the GX22-01 strain shares a high identity (94.3%-99.8 %) with aMPV/C isolates, especially with Chinese aMPV/C isolates from ducks and chickens, which are divided into aMPV/C cluster through N gene-based phylogenetic analysis. The aMPV/C GX22-01 strain was continuously passaged in Vero cells and the viral titers approximately reached 10