Kết quả định lượng progesterone huyết thanh khi đặt mẫu vòng tẩm progesterone vào âm đạo bò cắt buồng trứng=Result of quantifying progesterone content in serum when inserting progesterone releasing device in vagina of ovariectomized cows

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Tác giả: Văn Thanh Nguyễn, Thanh Long Sử, Tuấn Phong Vương

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2017

Mô tả vật lý:

Bộ sưu tập: Báo, Tạp chí

ID: 71793

Thêm vào giỏ

Progesterone releasing intravaginal devices are being studied and produced in Viet Nam in order to replace the importing progesterone releasing intravaginal devices into the country. This is neccessary for developing the Vietnamese cattle herd in general and Vietnamese dairy cows in particular. In this study, 4 ovariectomized cows were used as the experimental materials. The experiment was conducted in two stages, in the first stage intravaginal device containing 1,3 g was used in 11 days and in the second stage intravaginal device containing 1,9g progesterone was used in 7 days. The control device was CIDR (New Zealand). The gap between the first and the second stage was 2 months. ELISA method was applied to determine plasma progesterone concentration for all the experimental cows. The studied result showed that the plasma progesterone concentration was rapidly increased in blood of cow after inserting the device, and this progesterone concentration was maintained at high level in the whole period of inserting device then returned rapidly to the initial level after removing the device (PVòng tẩm progesterone đặt âm đạo bò đang được nghiên cứu sản xuất tại Việt Nam, nhằm chủ động nguồn cung loại vòng này để phát triển đàn bò nói chung, và bò sữa nói riêng nuôi ở ở ở nước ta. Nghiên cứu này đã thực hiện trên 4 con bò thí nghiệm đã cắt buồng trứng, đặt mẫu vòng tẩm progesterone vào âm đạo. Thí nghiệm được tiến hành trong 2 đợt đợt 1 đặt vòng tẩm 1,9g progesterone trong 11 ngày, đợt 2 đặt vòng tẩm 1,3 g progesterone trong 7 ngày. Mẫu đối chứng trong cả hai thí nghiệm là vòng CIDR (New Zealand), khoảng cách giữa 2 đợt thí nghiệm là 2 tháng. Phương pháp enzyme miễn dịch ELISA đã được sử dụng để định lượng progesterone huyết thanh. Kết quả thí nghiệm cho thấy lượng progesterone nhanh chóng được hấp thu vào máu của bò sau khi đặt vòng, hàm lượng progesterone trong máu được duy trì ở ở mức cao trong suốt thời gian đặt vòng, và nhanh chóng giảm xuống ngưỡng như ban đầu sau khi rút vòng khỏi âm đạo bò (P

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