Unraveling lipopolysaccharide-induced behavioral and molecular effects in Lymnaea stagnalis, an emerging model organism for translational neuroscience.

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Tác giả: Silvia Alboni, Cristina Benatti, Johanna M C Blom, Joris M Koene, Ken Lukowiak, Luca Pani, Giovanna Rigillo, Veronica Rivi, Fabio Tascedda

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Netherlands : International immunopharmacology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 718379

In this study, we employed a reductionist (yet not simplistic) approach utilizing the established invertebrate model system of the pond snail, Lymnaea stagnalis, to investigate the behavioral and molecular effects of systemic administration of lipopolysaccharide (LPS)-a bacterial endotoxin-on the snails' central ring ganglia. Snails received injections of either a low dose (2.5 μg) or a high dose (25 μg) of LPS, and their behavioral and molecular responses were assessed at 2, 6, and 24 h post-injection. With the high dose, snails exhibited a significant increase in homeostatic aerial respiration lasting for at least 24 h, consistent with a sickness-like state induced by the immune challenge. Additionally, we found that when administered 2, 6, or 24 h before operant conditioning training, the high dose of LPS, impaired memory formation. To further explore the underlying molecular mechanisms, we examined the transcriptional effects of the two doses of LPS in the snails' central ring ganglia. Our analysis showed a dose- and time-dependent upregulation of immune and stress-related genes, including key enzymes involved in the kynurenine pathway (KP), toll-like receptor 4 (TLR4), and heat shock protein 70 (HSP70). Metabolomic analysis suggested that the high LPS dose shifted KP metabolism toward the production of neurotoxic metabolites within the ganglia, indicating a LPS-induced neuroinflammatory state. Together, our findings provide valuable insight into the conserved mechanisms of neuroinflammation in this invertebrate model, offering a simplified yet effective tool to further explore the molecular interactions between the immune and central nervous systems.
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