Taking a massive overdose of methamphetamine (MAMP), which is one of the most prevalent illicit drugs in the world, will stimulate central nervous system, impair both physical and mental health and even lead to death. Therefore, the rapid quantitative testing in the field of MAMP is of great importance to law enforcement agencies. In this study, a branched, sharp-spiked gold nanostructure, named AuNS@4-MBA, is successfully synthesized at room temperature. The quantitative detection of MAMP is achieved through surface-enhanced Raman scattering (SERS) using the AuNS@4-MBA substrate. Due to the unique morphology of the nanostructure, numerous "hot spots" are formed, providing optimal conditions for sensitive detection. Upon activation of the system using EDC/NHS, the SERS signal intensity at 1080 cm