This study aims to evaluate the different processing methods, soaking-high-temperature pressure cooking (SHPC) and un-soaking-high-temperature pressure cooking (USHPC), on the quality of Basa (Pangasius bocourti) fish maw (BFM). Compared to traditionally cooked BFM, SHPC and USHPC treated BFM exhibited more severe disruption of collagen fibrils and network structure, significantly decreased texture, shear force and water holding capacity of BFM with the increase of HPC time, indicating that HPC could effectively reduce soaking time of BFM. Besides, as time HPC was 30 min, USHPC almost showed similar texture results to SHPC, and the former had the higher in vitro digestibility. Microstructural and proteomic analyses confirmed that SHPC displayed more exposed fibrous structures, with collagen fibers being more prone to be fragmentation and dissolution.