Transcriptomic profiles in major depressive disorder: the role of immunometabolic and cell-cycle-related pathways in depression with different levels of inflammation.

• Tác giả: Chiara Bottanelli, Edward T Bullmore, Annamaria Cattaneo, Jonathan Cavanagh, Philip J Cowen, Neil A Harrison, Melisa Kose, Veronika Kunšteková, Giulia Lombardo, Nicole Mariani, Moira Marizzoni, Valeria Mondelli, Maria A Nettis, Naghmeh Nikkheslat, Carmine M Pariante, Linda Pointon, Marco A Riva, Samantha Saleri, Luca Sforzini, Courtney Worrell, Zuzanna Zajkowska, Valentina Zonca
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Molecular psychiatry , 2025
• Bộ sưu tập: NCBI
• ID: 719606

Tóm tắt

 Transcriptomic profiles are important indicators for molecular mechanisms and pathways involved in major depressive disorder (MDD) and its different phenotypes, such as immunometabolic depression. We performed whole-transcriptome and pathway analyses on 139 individuals from the observational, case-control, BIOmarkers in DEPression (BIODEP) study, 105 with MDD and 34 controls. We divided MDD participants based on levels of inflammation, as measured by serum high-sensitivity C-reactive protein (CRP), in n = 39 'not inflamed' (CRP <
  1 mg/L), n = 31 with 'elevated CRP' (1-3 mg/L), and n = 35 with 'low-grade inflammation' (>
 3 mg/L). We performed whole-blood RNA sequencing using Illumina NextSeq 550 and statistical analyses with the Deseq2 package for R statistics (RUV-corrected) and subsequent pathway analyses with Ingenuity Pathway Analysis. Immunometabolic pathways were activated in individuals with CRP >
  1 mg/L, although surprisingly the CRP 1-3 group showed stronger immune activation than the CRP >
  3 group. The main pathways identified in the comparison between CRP <
  1 group and controls were cell-cycle-related, which may be protective against immunometabolic abnormalities in this 'non-inflamed' depressed group. We further divided MDD participants based on exposure and response to antidepressants (n = 47 non-responders, n = 37 responders, and n = 22 unmedicated), and identified specific immunomodulatory and neuroprotective pathways in responders (especially vs. non-responders), which could be relevant to treatment response. In further subgroup analyses, we found that the specific transcriptional profile of responders is independent of CRP levels, and that the inhibition of cell-cycle-related pathways in MDD with CRP <
  1 mg/L is present only in those who are currently depressed, and not in the responders. The present study demonstrates immunometabolic and cell-cycle-related transcriptomic pathways associated with MDD and different (CRP-based and treatment-based) MDD phenotypes, while shedding light on potential molecular mechanisms that could prevent or facilitate an individual's trajectory toward immunometabolic depression and/or treatment-non-responsive depression. The recognition and integration of these mechanisms will facilitate a precision-medicine approach in MDD.