BACKGROUND: Increasing evidence indicates a robust correlation between epilepsy and variants of the Kv7.2 ( METHODS: Whole-cell patch-clamp recordings, western blotting, and immunofluorescent staining were performed to elucidate the functional consequences of the identified variants. Moreover, coimmunoprecipitation techniques were conducted to explore protein interactions, thus facilitating a deeper understanding of the underlying pathogenetic mechanisms contributing to the disease. Ultimately, the effects of pharmacological interventions were evaluated in vitro using the patch-clamp technique. RESULTS: Herein, we identified 12 novel CONCLUSION: This study expanded the mutational spectrum of