Plants exhibit remarkable regenerative abilities under stress conditions like injury, herbivory, and damage from harsh weather, particularly through adventitious root formation. They have sophisticated molecular mechanisms to recognize and respond to wounding. Jasmonic acid (JA), a wound hormone, triggers auxin synthesis to stimulate root regeneration. Melatonin (MT), structurally similar to auxin, also significantly influences root induction, but its specific mechanism is unclear. Phytomelatonin's signal transduction is discovered in wound-induced root formation, identifying SlPMTR1/2 as phytomelatonin receptors, transmitting signals to SHOOT BORNE ROOTLESS 1 (SlSBRL1), a key regulator of wound-induced root regeneration, via the G protein α subunit 1 (SlGPA1). Additionally, SlPMTR1/2 is activated by JA, and targeted by SlMYC2. Overall, the specific mechanisms of phytomelatonin on wound-induced root regeneration is uncovered and revealed a crosstalk between phytomelatonin and JA, offering new insights into plant repair mechanisms.