Metabolite-Responsive Control of Transcription by Phase Separation-Based Synthetic Organelles.

• Tác giả: Carolina Jerez-Longres, Wilfried Weber
• Ngôn ngữ: eng
• Ký hiệu phân loại: 491 East Indo-European and Celtic languages
• Thông tin xuất bản: United States : ACS synthetic biology , 2025
• Bộ sưu tập: NCBI
• ID: 722048

Tóm tắt

Living natural materials have remarkable sensing abilities that translate external cues into functional changes of the material. The reconstruction of such sensing materials in bottom-up synthetic biology provides the opportunity to develop synthetic materials with life-like sensing and adaptation ability. Key to such functions are material modules that translate specific input signals into a biomolecular response. Here, we engineer a synthetic organelle based on liquid-liquid phase separation that translates a metabolic signal into the regulation of gene transcription. To this aim, we engineer the pyruvate-dependent repressor PdhR to undergo liquid-liquid phase separation