Iron-sulfur cluster proteins present the weak spot in non-thermal plasma-treated Escherichia coli.

• Tác giả: Julia Elisabeth Bandow, Ivan Bobkov, Tim Dirks, Marco Krewing, Britta Schubert, Kim Marie Weisgerber
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : Redox biology , 2025
• Bộ sưu tập: NCBI
• ID: 722581

Tóm tắt

Non-thermal atmospheric pressure plasmas have an antiseptic activity beneficial in different medical applications. In a genome-wide screening, hydrogen peroxide and superoxide were identified as key species contributing to the antibacterial effects of plasma while [FeS] cluster proteins emerged as potential cellular targets. We investigated the impact of plasma treatment on [FeS] cluster homeostasis in Escherichia coli treated for 1 min with the effluent of a microscale atmospheric pressure plasma jet (μAPPJ). Mutants defective in [FeS] cluster synthesis and maintenance lacking the SufBC