Salivary gland tumors (SGT) are a diverse group of tumors with various subtypes and morphological characteristics. Cell culture is a low-cost technique used as a valuable tool for studying cancer behavior and molecular characteristics. In vitro studies may offer a controlled environment for initial investigations before conducting in vivo experiments, making them indispensable in cancer research, drug testing, and personalized medicine. SGT cell culture techniques have been utilized to establish cell lines that provide insight into the genetic and molecular alterations underlying these tumors, aiding in the identification of potential therapeutic targets. Here, we highlight the application of cell culture techniques in studying SGT, emphasizing their contribution to advancements in understanding tumor behavior and potential novel therapeutic strategies.