Infection intensity and severity of Ranavirus transmission in juvenile wood frogs.

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Tác giả: Jesse L Brunner, Nicole C Dahrouge, Erin L Keller, Maya R Rayle, Tracy A G Rittenhouse

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Germany : Oecologia , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 722735

Ranaviruses are responsible for mass die offs of wood frog (Lithobates sylvaticus) tadpoles. What happens in between epidemics is less clear, but juvenile (metamorphosed) stages are hypothesized to move Ranaviruses among wetlands and introduce or reintroduce these viruses into wetlands, initiating new outbreaks. A key question is under what circumstances can juvenile L. sylvaticus infect susceptible conspecifics. We examined Ranavirus transmission between juvenile L. sylvaticus in two settings: first, we measured transmission from a Ranavirus-infected frog to a co-housed susceptible frog via cohabitation over a range of exposure periods. Second, we measured indirect transmission to susceptible frogs from a contaminated environment after a range of waiting times (i.e., from when the infected frog was removed to when the susceptible frog was exposed to the environment). We present evidence that juvenile frogs directly transmitted Ranavirus to susceptible frogs in all exposure periods (99.2% infected), with as little as 1 h of co-housing resulting in 95.8% of susceptibles infected. Indirectly, 96.8% of susceptible frogs became infected after as long as 48 h waiting times. Neither exposure period nor wait times influenced the probability of infection, because these probabilities are already high. In our linear regression models, susceptible frog viral load was significantly correlated with exposure period and cohort for the cohabitation experiment, while wait time was significantly correlated with viral load for the sequential habitation experiment. Collectively, our results suggest that Ranavirus transmission readily occurs between recently metamorphosed wood frogs and via terrestrial environmental exposure.
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