Chemotactic bacteria may overcome challenges posed by nonaqueous-phase liquid (NAPL) contaminants of low solubility in groundwater and limited bioavailability in tight pores by preferentially migrating to NAPL sources. We explored the transport of chemotactic bacteria to NAPL ganglia at varying pore water velocities in a dual-permeability microfluidic device and using computer-simulated solutions of transport equations. In our experiments, bacteria exhibited a chemotactic response toward NAPL ganglia at the junctures of low- and high-permeability regions (i.e., micropockets), and the extent of retention initially increased with velocity and then decreased at the highest velocity. A dimensional analysis revealed that maximum accumulations occurred at moderate values of the Péclet number