Single-Chain Nanobody Inhibition of Notch and Avidity Enhancement Utilizing the β-Pore-Forming Toxin Aerolysin.

• Tác giả: Jeremy Allred, Michael J M Anderson, Natalia S Babilonia-Díaz, Bruce R Blazar, Hannah N Bui, Peter M Gordon, Wendy R Gordon, LeeAnn Higgins, Benjamin Kopp, Andrew C D Lemmex, Joseph M Muretta, Jason Ostergard, Adam T Smiley, Oakley Streeter, Jake Trask
• Ngôn ngữ: eng
• Ký hiệu phân loại: 133.594 Types or schools of astrology originating in or associated with a
• Thông tin xuất bản: United States : ACS chemical biology , 2025
• Bộ sưu tập: NCBI
• ID: 723297

Tóm tắt

Notch plays critical roles in developmental processes and disease pathogenesis, which have led to numerous efforts to modulate its function with small molecules and antibodies. Here we present a nanobody inhibitor of Notch signaling derived from a synthetic phage-display library targeting the Notch negative regulatory region (NRR). The nanobody inhibits Notch signaling in a luciferase reporter assay with an IC