BACKGROUND: This survey study aims to evaluate knowledge of centrifuge operation and adherence to centrifugation protocols among laboratory personnel. These aspects are crucial in preventing pre-analytical errors. Additionally, the study seeks to assess the equipment infrastructure and staffing status within the participants' institutions. METHODS: The study involved 397 participants from various medical laboratories. A 16-item survey, using a 5-point Likert scale, was utilized to assess theoretical and practical knowledge of centrifuge operation. Two groups of subscales were developed, with one focusing on theoretical and practical knowledge and the other on pre-centrifugation, centrifugation, and post-centrifugation processes. Demographic data were collected, and the reliability of the survey was analyzed using Cronbach's alpha (0.94). Validity was assessed through factor analysis. RESULTS: The majority of participants were female (63%) and worked as laboratory technicians (72%). Approximately 71% had never received formal centrifuge training, and significant differences were observed between theoretical and practical knowledge, particularly among laboratory technicians (P <
0.001). Education level and job title also influenced knowledge scores (P <
0.001). CONCLUSION: The developed survey scale demonstrated high reliability and validity, indicating its potential as a valuable reference for future studies assessing centrifugation competency and usage in laboratory environments. These findings underline the need for improved training programs to reduce pre-analytical errors and enhance overall laboratory performance.