The ability to acclimate to changing environmental conditions is essential for the fitness and survival of plants. Not only are seasonal differences challenging for plants growing in different habitats but, facing climate change, the likelihood of encountering extreme weather events increases. Previous studies of acclimation processes of Arabidopsis thaliana to changes in temperature and light conditions have revealed a multigenic trait comprising and affecting multiple layers of molecular organization. Here, a combination of experimental and computational methods was applied to study the effects of changing light intensities during cold acclimation on the central carbohydrate metabolism of Arabidopsis thaliana leaf tissue. Mathematical modeling, simulation and sensitivity analysis suggested an important role of hexose phosphate balance for stabilization of photosynthetic CO