Understanding spatial variability in ecological and evolutionary patterns is key to Amazonian biodiversity conservation. This study examined taxonomic, phylogenetic, and functional diversity across amphibians and reptiles, assessing the influence of elevation, interrelationships among metrics, and distribution across five Amazon Basin ecoregions, exploring the "cradle" (speciation) and "museum" (lineage preservation) hypotheses. We analyzed 1011 amphibian species from three lineages and 828 reptile species from four lineages. Integrating distribution maps, phylogenies, and trait data, we calculated phylogenetic (PD), functional (FD), and taxonomic (TD) diversity, including mean phylogenetic (PD