Milk obtained from Yaks, yak hybrid (Dzomo) and hill cattle (Gauri) is major source of nutrients for people inhabiting Himalayas. In present study, QuEChERS approach together with HPLC-UV was used for detection and quantification of tetracycline residues in 170 raw milk samples of hill cattle (60), dzomo (58) and yaks (52) collected from high altitude regions of Himachal Pradesh, a Western Himalayan state of India. The method validated as per European Commission's guidelines was found to be linear (R