INTRODUCTION: Ethnic groups worldwide use mushrooms, particularly polypores (a group of fungi with woody fruiting bodies), to manage inflammatory conditions. In this study, the METHODS: Ethanolic extracts of the fungal materials were prepared and chemically characterized by UHPLC-ELSD/MS and TLC analyses before investigating the extracts' cytotoxic, antioxidant, anti-inflammatory, and lipid-stimulating properties. Radical scavenging and intracellular reactive oxygen species (ROS) assays were carried out in UVB-exposed human corneal epithelial (HCE-T) and immortalized human meibomian gland epithelial (IHMGEC) cells to evaluate antioxidant capacities. To examine the influence of the extracts of the inflammatory processes, associated with DED, a secretion assay for pro-inflammatory cytokines was conducted in UVB-exposed HCE-T and LPS-stimulated monocytic THP-1 cells. The lipid droplets secreted by IHMGECs were analyzed to determine the extracts' lipid-stimulating properties. RESULTS: Extracts of GT, GL, GO, and IO found to have high radical scavenging abilities. They significantly reduced intracellular ROS in UVB-exposed HCE-T and iHMGEC cells. GO and GL extracts inhibited cytokine secretion in HCE-T cells even at low concentrations. All tested extracts significantly inhibited the secretion of pro-inflammatory cytokines (IP10, IL-6, IL-8, and α) in LPS-stimulated monocytic THP-1 cells. CONCLUSION: Several extracts of the investigated fungal materials exhibit multifaceted pharmacological