Plant polyphenols are widely distributed in most higher plants, garnering significant attention from researchers due to their remarkable antioxidative, antibacterial, anticancer, and anti-radiation properties. They also offer multiple health benefits for various lifestyle-related diseases and oxidative stress. While there has been considerable research on the extraction and antibacterial application of plant polyphenols, developing a rapid and efficient extraction method remains a persistent challenge. Furthermore, the introduction of novel technologies is imperative to enhance the bioavailability of polyphenolic compounds. This comprehensive review synthesizes recent research findings pertaining to the extraction, antibacterial mechanisms, and applications of plant polyphenols. This research highlights the prevalent issues of low extraction rates of plant polyphenols and the ambiguous antibacterial mechanisms in current research. To address these challenges, this research proposes innovative directions for improving extraction technology and expanding antibacterial applications. Additionally, this review outlines promising future research avenues within the realm of plant polyphenols.