NanoASV: a snakemake workflow for reproducible field-based Nanopore full length 16S Metabarcoding amplicon data analysis.

• Tác giả: Laetitia Bernard, Arthur Cousson, Ulysse Guyet, Frédéric Mahé, Damase Razafimahafaly
• Ngôn ngữ: eng
• Ký hiệu phân loại: 917.3061 Geography of and travel in United States
• Thông tin xuất bản: England : Bioinformatics (Oxford, England) , 2025
• Bộ sưu tập: NCBI
• ID: 725959

Tóm tắt

SUMMARY: NanoASV is a conda environment and snakemake-based workflow using state of the art bioinformatics software to process full-length SSU rRNA (16S/18S) amplicons acquired with Oxford Nanopore Sequencing technology. Its strength lies in reproducibility, portability and the possibility to run offline, allowing in-field analysis. It can be installed on the Nanopore MK1C sequencing device and process data locally. AVAILABILITY: Source code and documentation are freely available at https://github.com/ImagoXV/NanoASV - Zenodo archive https://doi.org/10.5281/zenodo.14730742. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.