Effect of different protein ratios on in vitro digestive properties of soybean-wheat co-precipitated protein high-moisture extrudates.

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Tác giả: Xinru Cao, Liang Chen, Zhaoxian Huang, Lianzhou Jiang, Kunyu Ren, Mi Tian, Tian Tian, Xiaohong Tong, Huan Wang, Lexi Zheng

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Food chemistry , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 726594

The study examined the interdependencies between the interaction mechanisms and structural properties of soybean-wheat co-precipitated proteins high moisture extrudates (HME-SWCP) with different protein ratios during in vitro digestion and their digestion patterns. It was found that co-precipitation pretreatment resulted in higher digestibility, increased Zeta-potential, and reduced particle size after digestion. Digestibility of the extrudates increased and then decreased with higher wheat protein proportions. Notably, the ratio of soybean to wheat was 7:3, the HME-SWCP achieved the highest protein digestibility at 98.98 %. Rheological analysis showed viscosity initially increased and then decreased with more wheat protein. Amino acid analysis and SDS-PAGE revealed HME-SWCP digestion products had 1.15 to 1.57 times more amino acids than HME-SPI and a lower abundance band. FTIR analysis showed a shift from folded to α-helix structure post-digestion, with decreased random coil content. Increased fluorescence intensity and changes in disulfide bonds suggested HME-SWCP is more susceptible to enzymatic degradation, facilitating absorption. This study provides valuable insights for improving the nutritional profile of plant-based meat products.
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