The enhanced effect and mechanism of endogenous sigma factor RpoF on bioelectricity generation in Pseudomonas aeruginosa-inoculated Microbial fuel cells (MFCs).

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Tác giả: Hongchen Jin, Jianmei Luo, Zhengshu Niu, Wanjun Tian, Tingting Wang, Jingmei Zhang, Minghua Zhou

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Biosensors & bioelectronics , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 726600

Microbial fuel cells (MFCs) has attracted tremendous attention due to integrating clean energy generation and wastewater treatment. Electricigens are in charge of electron transfer and energy conversion, and therefore strain improvement is crucial for MFCs performance. Herein, the overexpression of sigma factor RpoF and the combined manipulation with other regulators (PmpR and RpoS) reinforced electricity generation of a model strain Pseudomonas aeruginosa PAO1. Next, RpoF was introduced into an isolate P. aeruginosa P2-A-12 with higher electroactivity, which not only yielded 3.2-fold increase in the maximal power density under non stress, but also generated 21.4 % improvement under 1.5 % NaCl. The comprehensive analysis at the levels of cells, metabolites and genes transcription ascertained its global regulatory mechanism, mainly including the enhanced biofilm formation by promoting cell attachment and cell-to-cell adhesion on the anode, more c-di-GMP and quorum sensing (QS) signal molecules accumulation, and the increase in phenazine-1-carboxamide (PCN), pyocyanin (PYO) and 1-hydroxyphenazine (1-OHPHZ) by controlling the expression levels of multiple genes involved in core biosynthesis and QS system. It was the first time to demonstrate the direct activation of RpoF on phzH responsible for PCN production and rhlR regulating N-butanoyl-HSL (C
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