OBJECTIVES: Carbapenems are considered to be the last resort to the serious infections caused by multidrug-resistant (MDR) Gram-negative bacteria. The emergence of carbapenem-resistant Enterobacteriaceae has posed serious threat to human health. However, carbapenem resistance is rarely reported in E. fergusonii. In this study, an NDM-5 producing E. fergusonii strain EFSXRJ10 was isolated from chicken in China. METHODS: The minimal inhibitory concentrations (MICs) were determined using broth microdilution-based antimicrobial susceptibility testing. The complete genome sequence of the NDM-positive isolate was obtained through Illumina NovaSeq and Oxford Nanopore GridION sequencing platforms, followed by hybrid assembly with Unicycler. In the plasmid conjugation assay, the sodium azide-resistant E. coli strain J53 was employed as the recipient. RESULTS: Strain EFSXRJ10 was resistant to ampicillin, amoxycillin-Clavulanic acid, gentamicin, spectinomycin, tetracycline, florfenicol, sulfafurazole, cefotaxime, ceftazidime, apramycin and meropenem. The bla CONCLUSIONS: This is the first report on the characterization of bla