The Diagnostic Efficacy of Metagenomic Next-Generation Sequencing (mNGS) in Pathogen Identification of Pediatric Pneumonia using Bronchoalveolar Lavage Fluid (BALF): A Systematic Review and Meta-Analysis.

• Tác giả: Li Ai, Lingdan Chang, Mengli Fu, Bing Hai, Ran Li, Rui Li, Zhifang Ruan, Hongjin Shi, Yan Wang, Yuan Yang, Dan Zeng, Jinsong Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 922.945 *Hindus
• Thông tin xuất bản: England : Microbial pathogenesis , 2025
• Bộ sưu tập: NCBI
• ID: 727205

Tóm tắt

 OBJECTIVE: This meta-analysis evaluates and compares the diagnostic efficacy of metagenomic next-generation sequencing (mNGS) with conventional microbiological tests (CMTs) in diagnosing pediatric pneumonia using bronchoalveolar lavage fluid (BALF). METHODS: Data were sourced from Embase, Medline (via PubMed), Web of Science, and the Cochrane Library. The pooled positive detection rate (PDR) of pathogens was estimated using fixed-effects or random-effects models. Subgroup analyses explored factors influencing mNGS diagnostic performance. Data analysis was conducted using Review Manager (RevMan) 5.4 and Stata version 16.0. RESULTS: The pooled PDR of mNGS was 85.83%, which is higher than the pooled PDR of 49.97% for CMTs. A random-effects model indicated that, compared to CMTs, mNGS has a significantly higher PDR in pediatric pneumonia (OR=3.99, 95%CI: 2.12-7.50, P<
 0.0001, I