Antibiotic resistance genes （ARGs） as a novel type of pollutant can lead to the ineffectiveness of antibiotics used in clinical treatment. The environment serves as an important reservoir for ARGs, and therefore, the migration and transformation of ARGs in the environment have attracted increasing attention in recent years. However, studies on the presence of ARGs in rural drinking water environments remain scarce. This study focused on Henan Province, a major agricultural province, where 35 samples of rural drinking water were collected and quantitatively analyzed for 16 types of ARGs belonging to seven classes, as well as the integrase gene