Simultaneous nitrification and denitrification (SND) of nitrogen-rich wastewater in microbial fuel cells (MFCs) is a new-age technology capable of treating wastewater and concurrently generating bioelectricity. Compared to the conventionally used biological nitrogen elimination processes, SND in MFC is much more energy and cost-efficient because it uses less organic carbon and excludes the nitrified liquid circulation process. In this work with a dual-chambered MFC, carbon-rich synthetic wastewater (CRSW) with an invariable glucose concentration of 2 g/L has been treated in the anodic chamber and nitrogen-rich synthetic wastewater (NRSW) containing 1 g/L, 2 g/L, and 3 g/L ammonium chloride (NH